Serial Dilution Calculator

About Serial Dilution Calculator

Final concentration and volumes for a dilution series. The Serial Dilution Calculator on Picscaler gives you the answer instantly in your browser, with no sign-up.

Inputs: Starting concentration, Dilution factor per step, Number of steps and Final volume per tube (mL). Results shown: Total dilution factor, Final concentration, Transfer per step and Diluent per tube.

Formula: Total dilution = factor^steps; final concentration = start / total dilution; transfer = tube volume / factor. Concentration is in the same unit you entered.

This tool is for education and information. It does not replace clinical judgement, so confirm results against your laboratory's reference ranges and local guidelines.

How to use Serial Dilution Calculator

1. Enter Starting concentration, Dilution factor per step, Number of steps and Final volume per tube (mL).
2. Press Calculate.
3. Read Total dilution factor, Final concentration, Transfer per step and Diluent per tube in the result table.
4. Use the formula and notes under the result to check the working.

Frequently asked questions

Is Serial Dilution Calculator free?

Yes. Serial Dilution Calculator is completely free to use on Picscaler.

Do I need to create an account?

No. You can use it straight away without signing up.

Is my data safe?

Your input is processed in your browser on your own device and is not uploaded to Picscaler.

Can I use it for medical decisions?

No. It is an educational aid and does not replace professional medical judgement.